番鸭呼肠孤病毒人工感染雏番鸭肝的蛋白质组学分析

doi:10.11843/j.issn.0366-6964.2017.10.018

Abstract

Abstract:

The purpose of this paper was to study the pathogenesis of Muscovy duck reovirus (MDRV) infected muscovy ducking. Forty-four healthy muscovy ducklings were divided into treatment group challenged with MDRV and control one injected with Hank's. Livers were collected at the 5^th day after challenge. Proteomics of the livers were determined by differential proteomic technology. Differential protein spots between both groups were performed and analyzed by two-dimensional gel electrophoresis and MALDI-TOF-TOF mass spectrometer, and then they were identified by protein search software, Mascot 2.3.02. Thirty-eight differential protein spots were separated and identified. Ten down-regulated protein spots and seventeen up-regulated protein spots were observed in the liver samples of the treatment after comparison between the two groups. Seven protein spots were appeared only in the livers of control and four protein spots were only emerged in the livers of treatment. The results indicate that differential proteomics in livers, which were impacted and damaged by MDRV may provide a new idea of pathogenesis of MDRV infected muscovy duckling.

CLC Number:

S852.659.4

ZHU Guo-zhen, HUANG Mei-qing, CHENG Xiao-xia, ZHENG Min, CHEN Shi-long, CHEN Shao-ying, YU Fu-song. Proteomics Analysis in Livers of Muscovy Ducklings Infected by Muscovy Duck Reovirus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(10): 1949-1957.

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Proteomics Analysis in Livers of Muscovy Ducklings Infected by Muscovy Duck Reovirus

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